الفهرس 
IntroductionOnly 14 pages are availabe for public view
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Abstract
Background: Faba bean (Vicia faba L.) is considered to be one of the most oldest crops grown by man and is known as the poor man`s meat. The cultivation importance of faba bean around the world based on its high nutritional value of protein (about 28% in dried seed), carbohydrates (56%), vitamins and some other compounds. Also, faba bean improves the soil fertility due to its ability to fix nitrogen. Among all faba bean diseases, chocolate spot is the most important one worldwide which can be seed-borne or residue-borne. Estimation of losses in yield due to chocolate spot disease in the Nile Delta may range 60-80% among susceptible cultivars, and up to 34% among the resistant ones. Aim: This study aimed to evaluate the efficacy of some biological control agents such as Trichoderma fungi, Rhizobium leguminosarum and Saccaromyces cerervisiae yeast for biocontrol of chocolate spot disease of Vicia faba L. plant. Methods: Samples of naturally infected Vicia faba leaflets that showed typical symptoms of chocolate spot disease were collected from five fields inside each district of five different districts in Dakahlia governorate. The most aggressive isolate Botrytis fabae B4 and the most potent antagonistist Trichoderma harizianum E3 and Trichoderma viride T2, plus R. leguminosarum and S. cerevisiae, were selected through laboratory experiments for further studies of greenhouse and field experiments. Results: from our results, the bioagents used in this study (Rhizobium leguminosarum, Trichoderma harzianum, Trichoderma viride, and Saccharomyces cerevisiae) as individual or their combinations succeeded in increasing the resistance of infected faba bean plant against chocolate spot disease caused by Botrytis fabae as well as improving the yield production and increasing plant growth parameters, enhancing physiological aspects and leaflet structure. Conclusion: Faba bean seed inoculation accompanied with foliar spraying of trio-combination with Rhizobium leguminosarum, Trichoderma viride and Saccharomyces cerevisiae during the vegetative growth stage twice (35 and 55 days after sowing) lead to decreasing the harmful effect of chocolate spot disease and achieve the highest seed yield as well as improved seed quality of faba bean.. So, these bio-agents could be easily used as alternatives to the used chemical pesticides.