الفهرس 
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Abstract
Lymphoid leukosis (LL) is a B cell tumor of the chicken bursa with a huge economic impact on the poultry industry. The tumors are induced by infection with Avian Leukosis Virus (ALV). However, endogenous ALVs is known to have little or no oncogenic potential. Marek’s disease (MD) is another viral neoplastic disorder with hogh mortality rate in chicken. Serotype 2 MD vaccine (MDV2) is an attenuated virus and naturally non-oncogenic but has been shown to enhance the development of both exogenous ALV-induced and spontaneous lymphoid leukosis. AF-227 is a new field strain of subgroup E endogenous ALV (ALV-E). AF-227 has been isolated from commercial chicken experiencing spontaneous ALV-like LL. Although ALV-E viruses are known to be non-oncogenic, the influence of ALV-E and its possible interaction with MDV2 on the enhancement of spontaneous ALV-like LL are still unclear. In this study we used RNA-Seq to generate an expression profile from spontaneous ALV-like LL obtaine from chickens inoculated with strain AF-227 of ALV-E in conjunction serotype 2 MD vaccine to uncover potential molecular oncogenic events.. We identified the absence of Tumor suppressor candidate 2 (TUSC2 ) in all tumor samples. TUSC2 is a tumor suppressor gene which is considered as a molecular link between inflammatory response and mitochondrial homeostasis (Hood et al., 2013; Uzhachenko et al., 2012). TUSC2 can influence and complement the PI3K/AKT and p53 pathways. Our pathway enrichment analysis showed significant dysregulation of both pathways. We also identified overexpression of another important proto oncogene called Eukaryotic.