الفهرس 
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Abstract
Sargassum latifolium was collected from the Red Sea coast. Sodium alginate from S. latifolium thallus yielding 17.5% w/w. Chemical characterization of S. latifolium is rich in total sugars (41.08%) and uronic acids (47.4%); while, proteins, lipids and sulfates contents are 4.61, 1.13 and 0.09%, respectively. UV analysis was performed indicating the presence of proteinaceous constituents, phenolic constituents and flavonoids. NMR, FTIR and TGA analyses were also performed. Crystallinity index (0.334) indicates alginate semicrystalline nature. Sodium alginate hydrolysate was evaluated as Chlorella vulgaris growth promoter. The highest stimulation (0.7 g/L biomass) was achieved by using 0.3 g/L alginate hydrolysate supplementation. The highest total soluble proteins and total carbohydrates in response to 0.3 g/L alginate hydrolysate were 179.22 mg/g dry wt and 620.33 mg/g dry wt, respectively. The highest total phenolics content (27.697 mg/g dry wt.), guaiacol peroxidase activity (2.899 μmol min-1 g-1) were recorded also to 0.3 g/L alginate hydrolysate supplementation. • Sodium alginate was used for preparation of Riboflavin-entrapped barium alginate-Arabic gum formulations exhibited prolonged riboflavin release over 120 min in simulated gastric fluid. MG biosorption depends on various influences as, pH, incubation time, temperature, alginate concentration and initial MG concentration. The maximum MG bioremoval percentage occurred at pH 6, 35⁰C, 0.6 g L-1 alginate concentration and 35 mg L-1 MG concentration at 5 hours incubation time. Face centred central composite design (FCCCD) was applied to assess the most considerable factors for MG bioremoval from waste water using alginate beads. Predicted data revealed that 0.8 g L-1 alginate beads removed 91.53% of MG from the polluted solution using 40 mg L-1 MG concentration with shaking at 150 rpm and pH 8 after 7 hours. Alginate beads have the ability to remove MG from aquatic wastewater efficiently.