الفهرس 
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Abstract
Preparing silver nanoparticles solution using Rumex dentatus extract as a reducing agent with AgNO3 (0.68 mg/L) under UV light for 20 min.Characterization of AgNPs using Transmission Electron Microscope and UV analysis.Testing anti-microbial activity of three serial dilution of AgNPs, AgNO3 (0.68, 0.068, 0.0068 and 0.00068) mg/L and R. dentatus extract solution with Total Bacterial Count method using a wastewater sample collected from both study areas (Samaha and Mansoura stations), resulting in complete reduction of bacterial growth for 7 days of AgNPs original preparation, AgNO3 (0.68 mg/L) and no effect of R. dentatus extract. Testing Coli form count in both study areas samples using AgNPs with complete bacterial growth removal after 24h. Preparation of free, laden chlorella minutissima and laden AgNPs and chlorella minutissima calcium alginate microbeads and characterized by Scanning Electron Microscope. Alginate microbeads free and laden C. minutissima size ranged between 4 to 15 nm, while alginate microbeads laden AgNPs and C. minutissima size ranged between 10 to 25 nm. Wastewater two samples collected from each station (Samaha and Mansoura station) before chlorination stage at winter 2019 and spring 2021 with performing chemical, physical and bacteriological analysis. The nitrate, nitrite, ammonia and SRP analysis for Samaha wastewater samples in winter 2019 were 0.212, 0.03, 36 and 4.723 mgl-1, while in spring 2021 were 0.215, 0.035, 35.6 and 5.321 mgl-1 respectively. The nitrate, nitrite, ammonia and SRP analysis for Mansoura wastewater samples in winter 2019 were 2.2, 0.712, 10.34 and 4.423 mgl-1, while in spring 2021 were 3.1, 0.615, 10.5 and 5.312 mgl-1 respectively. Total bacterial count was investigated also in Samaha and Mansoura during winter 2019 and spring 2021, for Samaha was 320x1010 and 300x1010, while Mansoura was 300x109 and 310 x109 respectively. This study concluded that alginate microbeads laden Chlorella minutissima has the most effective removal for the wastewater in both studying area, however microbeads laden chlorella minutissima and AgNPs preferred due to the facility of AgNPs removing from treated water.