الفهرس 
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Abstract
”Nutritional problems related to rumen acidosis in Cattle A total number of 210 dairy cattle, belonged to 12 different farms in Dakahlia governorate, Egypt was included in this study. Of which, 30 apparently health cattle were served as a control group, besides 180 cattle supposed to clinically suffer SARA. The examined cattle were aged (26±5) months old and weighed (560±20) Kg. The average values of cattle’ body condition scores (BCS) were (3.03 ± 0.07), using 1 to 5 scale degrees). Generally, all cattle were studied during the first 60 days of lactation. I-Risk factors investigation In this regard, data were recorded and categorized into animal level including; age of cattle, breed, and daily milking production. In addition to the farm level that involved the feeding regimen, feed additives, impact of mycotoxins and the milking production routine. All investigated parameters were recorded and given scores for further statistical analysis. II-Ration Analysis In current study, the ration was analyzed protein percentage, calcium, phosphorus, energy and ash were analyzed. The (VICAM) Instrument Calibration for rapid food analysis for mycotoxins (Ocratoxins & Aflatoxins). III-Blood Sampling Individual blood samples (10mL) were collected from all studied dairy cows from the jugular vein, where (7mL) of the collected blood were received in anticoagulant free tubes to get sera needed for the biochemical examination, while the other blood sample (3mL) was kept in sodium fluoride tubes for rapid glucose analysis. Additionally, (3mL) blood samples were collected from all investigated cows via the tail coccygeal artery using ventilated syringes with 23 G × 1 needle, containing freeze-dried lithium heparin for blood gas analysis. IV. Serological analysis of biochemical analytes Serum samples were separated by centrifugation at 3000 r.p.m for 10 minutes. The clear sera were received in dry sterile sample tube using sterilized pipettes, processed directly for assessing glucose concentration and the enzymatic activities of liver markers; alanine transaminase (ALT), and aspartate transaminase (AST). Furthermore, serum triglycerides (TG) and total cholesterol concentrations were measured. Additionally, the non-esterified fatty acid (NEFA), β-hydroxybutyrate (β-HBA) and high density lipoprotein (HDL) concentrations were estimated. Finally, the acute phase proteins; albumin, haptoglobulin (Hp), serum amyloid A (SAA) and the C-reactive protein (C-RP) were measured. V. Treatment prebiotics on the outcome of SARA, next generation prebiotics powder (Ecocell prebioticsR) containing derived cell wall of saccharomyces cerevisiae, with Mannan Oligosaccharides (MOS) enforced by extract of the root of chicory, β glucans and inulin (FOS), was used in a dose of 0.5kg/Ton in feed. Moreover, flash start- spirulina powder (250gm) was used in a dose rate of 1kg/Ton, added to feed for dairy cattle. ”