الفهرس 
Introduction and Aim of the workOnly 14 pages are availabe for public view
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Abstract
Inflammatory bowel disease (IBD) is characterized by chronic intestinal inflammation due to dysregulation of the mucosal immune system. Although inflammatory bowel diseases (IBDs) are associated with a high risk of colon carcinogenesis, the mechanisms underlying this association have not yet to be fully elucidated. Colitis is one of the most prevalent health problems worldwide as it makes patients’ lifestyle difficult as well as the medical treatments have unfavourable side effects. So one of the important target aims to find natural compounds for preventing and treating this disease. Frankincense is one of the most widely used medicinal plants for the treatment of inflammatory diseases. Therefore, the current study aims to demonstrate the benefits of fractions of Boswellia extract in treating colitis and investigate its mechanism of action. the current study was conducted in two phases to achieve its objectives :ر- 1. 1st phase of the study was conducted in the laboratory as follows : Separation of frankincense extract components into four fractions s (boswellia acids - Oleoresin - oil - resin). Studying the effect of any of the four extracted components on cancer cell lines (breast MCF-7, liver HepG2 colon Ht-29) and normal cells as HSF. The results of the first phase were obtained as following : 1. Oleoresin fraction increased the viability of colon cells (HT-29) and breast cells (MCF-7) compared to other fractions of the extract. 2. Oil fraction has the greatest effect on the vitality of HepG2 cell line. 3. All extract fractions decreased the viability of the studied cell lines at concentration of 100 µg/ml, except resin fraction increased the viability of these cells. 4. All extract fractions decreased the vitality of normal skin cells HSF, except for the resin. Based on the in vitro results of the effects of different fractions of Boswellia extract, oleoresin fraction has been selected to investigate its effect on experimental colitis induced by DSS. 2. 2nd phase of the study was conducted on BALB/c mice as follows : Sixty male BALB/c mice of approximately the same weight 25-30 g were used in this study. They were divided into six equal groups (10 mice each) and these groups were treated according to the following : 1. Control group (G 1): Healthy mice that did not receive any treatment. 2. Normal treated group with 50 mg oleoresin extract (G2): Healthy mice were administered orally with 50 mg extract / kg body weight once daily for 7 days. 3. DSS-induced colitis group (G3) : A group of healthy mice received 3% DSS daily in drinking water for 7 days for induction of acute colitis 4. DSS-induced colitis group (protected model) (G4): A group of healthy mice received 3% DSS daily in drinking water for 7 days for induction of acute colitis and oleoresin fraction dissolved in 0.5% carboxymethyl cellulose (CMC) orally with a dose 50 mg/kg/day once daily for 7 days. 5. DSS-induced colitis (G5): A group of healthy mice received 3% DSS daily in drinking water for 7 days for induction of acute colitis. 6. DSS-induced colitis group (treated model) (G6): A group of healthy mice received 3% DSS daily in drinking water for 7 days for induction of acute colitis and then after induction of colitis all mice treated with oleoresin fraction dissolved in 0.5% carboxymethyl cellulose (CMC) orally with a dose 50 mg/kg/day once daily for 7 days. At the end of treatment period, the animals were left one day before scarifying to obtain serum and colon for estimation some markers and compare their levels in various previous experimental groups and the following parameters were determined.