الفهرس | Only 14 pages are availabe for public view |
Abstract Escherichia coli is one of the most important causes of foodborne outbreaks, as it causes serious diseases as HUS and HC. These microbes can spread on the carcasses and internal organs of slaughtered animals and transmit to human through meat causing health problems. This study was done to determine the incidence, serotypes, presence of virulence genes and antimicrobial resistance of Escherichia coli isolates isolated from camel meat. 123 fresh camel meat samples (250 gm for each) were taken from an abattoir at Elbeheira governorate, Egypt. The results of this study revealed that out of 123 camel meat samples randomly taken from different carcasses at municipal slaughterhouse at Elbeheira province, Egypt E. coli was detected in 41.4% (51/123) of tested samples based on molecular confirmation using PCR technique. 92 isolates were serologically identified as E. coli, 46 out of 92 isolates were identified as shigatoxin producing E. coli. The results of the serological identification showed that there were 10 different serotypes of Escherichia coli. The present study indicated that all (100% ; 92/92) serologically identified E. coli isolates examined were molecularly confirmed by multiplex PCR based on the presence of stx1 (614 bp), stx2 (779 bp), eaeA (890 bp) and hlyA (165 bp) genes. All Escherichia coli isolates recovered from camel meat samples were tested for antimicrobial resistance against 14 antimicrobial agents and showed different resistance. |