الفهرس 
INTRODUCTIONOnly 14 pages are availabe for public view
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Abstract
This research aims mainly at the effectiveness of extracts of some medicinal plants in controlling and treating pathogenic germs in an attempt to reduce the need for drugs with dangerous and harmful side effects on humans. A total of 150 clinical samples were collected during the 2021 year from Mansoura University Hospital. The clinical samples included blood (20), urine (75), and stool (55) from different patients with different genders and ages ranging from 16 to 55 years. The studied isolates were different from others in colony morphology. A large variety of both Gram-negative and Gram-positive bacteria are involved. This bacterial species is a member of the ESKAPE group recently described as the main contributor to the health human infection problem. In this study, the obtained isolates were biochemically characterized according to enzymatic activity and function properties. Through the biochemical descriptions of these isolates, they all show their ability to produce some enzymes such as catalase enzymes, amylolytic, proteolytic, lipolytic enzymes and motility. All the bacterial isolates indicated negative results to produce indole; on the other side all the bacterial isolates indicated negative results to produce hydrogen sulphide except Salmonella typhi indicated positive results. The qualitative analysis showed that plant samples were rich in varying amounts of alkaloids, flavonoids, phenols, saponins, and tannins. In this work, the antioxidant properties of four selected plant extracts (A. javanica, E. retusa, H. salicornicum and L. nudicaulis) were in vitro evaluated by DPPH assay. Based on the results of IC50, the antioxidant activity of the four plant samples follows the sequence L. nudicaulis (57.68 mg/ml) ˃ E. retusa (62.27 mg/ml) ˃ H. salicornicum (71.35 mg/ml) ˃ A. javanica (90.98 mg/ml). All the tested extracts have shown antioxidant scavenging activities but with values higher than that of catechol (IC50 = 15.23 mg/ml).The cytotoxic activities of the prepared plant samples extracts were evaluated using an MTT assay. The samples were tested in vitro against two tumor cells, i.e., HepG-2, and PC3 cell lines. Doxorubicin was selected as a reference drug, comparing the results of the tested samples against the different cancer cells. According to the results, the methanolic extracts of plants exhibited cytotoxic activity in a dose-dependent manner, which was comparable with doxorubicin as a reference standard. Based on the results of IC50, the cytotoxic activity of the four plant samples follows the sequence E. retusa ˃ L. nudicaulis ˃ H. salicornicum ˃ A. javanica for two selected human tumor cells.